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KMID : 0380620180500040437
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Korean Journal of Food Science and Technology
2018 Volume.50 No. 4 p.437 ~ p.443
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Immunomodulatory effect of bee pollen extract in macrophage cells
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Kim Yi-Eun
Cho Eun-Ji
Byun Eui-Hong
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Abstract
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Activation of macrophages plays an important role in the host-immune system. In this study, we investigated the functional roles and related signaling mechanism of hot-water extracts of bee pollen (BPW) in RAW 264.7 macrophages. Since BPW did not exert cytotoxicity at concentrations ranging from 62.5 to 250 μg/mL in macrophage cells, a concentration of 250 μg/mL was used as the maximum dose of BPW throughout subsequent experiments. BPW increased inducible nitric oxide synthase-mediated nitric oxide production in a concentration-dependent manner. Additionally, BPW was found to induce macrophage activation by augmenting the expression of cell surface molecules (cluster of differentiation; CD80/86, and major histocompatibility complex; MHC class I/II) and production of proinflammatory cytokines (tumor necrosis factor-α, interleukin-6, and IL-1β) through mitogen-activated protein kinase and nuclear factor-κB signaling pathways in RAW 264.7 macrophages. Taken together, our results indicate that BPW could potentially be used as an immunomodulatory agent.
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KEYWORD
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bee pollen, macrophage, immuno-modulatory activity, cytokine production, mitogen-activated protein kinase
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